Get Full Essay Get access to this section to get all help you need with your essay and educational issues. I contribute to planning by giving verbal and written feedback as well as observing while children play and communicate to see how well the children have done on an activity.
What is an autoclave? Pressure steam sterilisers autoclaves are used in laboratories both for sterilisation of media and equipment required for the culture of microorganisms, and for sterilisation of discarded cultures and waste materials. Pressure steam sterilisers operate at high pressures and temperatures, and appropriate measures must be taken for to ensure the safety of workers.
Autoclaves utilise moist heat, in the form of saturated steam under pressure, to destroy microbial life. Steam sterilisation is the most reliable sterilisation method for the majority of situations.
The autoclave is used to sterilise: The following elements all contribute to autoclave effectiveness. The time required for sterilisation varies, but it will never be less than 30 minutes. The time is measured from the point at which the temperature of the material to be sterilised reaches the required autoclaving temperature - the tighter the autoclave is packed, the longer it will take for the material in the centre of the load to reach the temperature required.
Saturated steam must contact all areas of the load. Sterilisation will fail if: Autoclaves must be used properly to effect successful steam sterilisation. What are the general guidelines for ensuring autoclave effectiveness? There are a number of general guidelines that should be followed when using autoclaves to ensure effective sterilisation.
Indicators, such as autoclave tape, indicate with a visible colour change that they have been through the autoclave process. At the end of the autoclave cycle, indicators should be checked to ensure they have changed colour.
There are commercially available test kits that use bacterial spores to test the autoclave efficiency. Steam and heat cannot easily penetrate a densely packed autoclave bag.
If an autoclave is overfilled the outer contents of the bag will be sterilised, but the innermost part will be unaffected.
An over packed autoclave chamber does not allow efficient steam distribution, and so sterilisation efficiencies will be reduced. Autoclave temperature, pressure and cycle duration time should be monitored during each cycle. Some autoclaves have charts that trend the temperatures and pressures inside the autoclave chamber throughout each cycle.
Autoclaves must be covered by a regular preventative maintenance program that is performed by a technician certified by the manufacturer.
Most items to be autoclaved are packed into autoclave bags, which come in a variety of types and sizes. Paper autoclave bags Paper autoclave bags are commonly used when autoclaving reusable equipment after it has been disinfected and washed. Paper autoclave bags should be closed by folding down and taping the open end as they are permeable to steam.
Plastic autoclave bags Plastic biohazard bags are commonly used when autoclaving biological waste before disposal. Autoclave bags should be left partially open, to allow steam to penetrate the bag.
Where possible, water should be added to autoclave bags to facilitate saturated steam contact. What do I need to know when autoclaving without an autoclave bag?
Some reusable equipment is autoclaved without autoclave bags, and in this instance the following guidelines should be followed. Tubular equipment such as pipettes and equipment such as conical flasks should be plugged with a wad of cotton wool, so that the interior of the equipment remains sterile after autoclaving.The products shall be tested in accordance with tests and examinations contained in the 15th edition of Official Methods of Analysis of the Association of Official Analytical Chemists or in the 15th edition of Standard Methods for the Examination of Dairy Products.
The ADA is a comprehensive civil rights law. It prohibits discrimination on the basis of disability in employment, state and local government programs, public accommodations, commercial facilities, transportation, and telecommunications.
Most resources will be available but you may require to purchase them from a catalogue or online otherwise. Ensure the learning environment meets relevant health, safety, security and access . to manage their environment to ensure the health and safety of themselves and others; to explain the steps they take to control risks.
In addition to this general teaching requirement for the five subjects, the programmes of study for each subject contain specific teaching requirements on .
Ensure the learning environment is safe and free of hazards during practical activities; Ensure equipment and materials are used properly to support the student’s learning needs; Ensure equipment and materials are stored in a safe manner when not in use; Assist in implementing appropriate safety procedures in .
• The learning environment is inspected prior to use and in consultation with Element 4 - Develop and Implement Actions to Ensure the Health Safety and Welfare of Learners and/or Candidates relevant .